Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Biglycan Double Nickase Plasmid (h): sc-401408-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Biglycan Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Biglycan Double Nickase Plasmid (h) and Biglycan Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BGN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Biglycan Antibody (3E2): sc-100857
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Biglycan Double Nickase Plasmid (h) | sc-401408-NIC | 20 µg | $410.00 | |||
Biglycan Double Nickase Plasmid (h2) | sc-401408-NIC-2 | 20 µg | $410.00 |
BGN encodes biglycan, a small leucine-rich proteoglycan of the extracellular matrix that regulates collagen fibrillogenesis, matrix organization, and tissue biomechanics. Biglycan binds growth factors and cell-surface receptors, influencing signaling pathways such as TGF-β, Wnt/β-catenin, and toll-like receptor–mediated inflammatory responses that shape cell adhesion, migration, and differentiation. Altered BGN expression and proteoglycan remodeling are linked to fibrosis, vascular and cardiac remodeling, osteoarticular degeneration, and tumor–stroma interactions, making BGN a useful node for studying ECM-driven cell signaling and mechanobiology. In human systems, BGN is frequently used as a marker and functional mediator of stromal activation and matrix-associated inflammatory cues.
Biglycan Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BGN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BGN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BGN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BGN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.