Thank you for your question. Our CRISPR knockout products are designed to have only a single binding site within their target gene. However, despite this, unpredictable off-target effects may occur.
If the setup of your experiment cannot tolerate off-target effects, you may want to opt for a double nickase product instead. The mechanism they rely on makes the occurrence of off-target effects virtually impossible.
Fecha de publicación: 2021-02-09