BECN1/Beclin-1 Antibody (G-11): sc-48381

Beclin 1 Antibody (G-11) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect Beclin 1 across mouse, rat, and human species, making anti-Beclin 1 antibody (G-11) an indispensable reagent for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Beclin 1 monoclonal antibody (G-11) is raised against amino acids 1-300 of human Beclin 1 and is available in both non-conjugated form and multiple conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental requirements. Beclin 1, also called BECN1, ATG6, VPS30, and beclin1, is a crucial coiled-coil protein primarily localized in the cytoplasm, where Beclin 1 associates with membranes of the endoplasmic reticulum, mitochondria, and the Golgi apparatus to orchestrate autophagosome formation, a fundamental step in the autophagy pathway. Autophagy is essential for cellular homeostasis, enabling the degradation and recycling of damaged proteins and organelles, and plays a significant role in preventing uncontrolled cell growth and tumorigenesis. By interacting with Bcl-2, Beclin 1 regulates autophagic activity, thereby maintaining cellular integrity and responding to stress conditions. Beclin 1′s cellular localization coordinates the spatial and temporal aspects of autophagosome formation, which is critical for effective autophagic flux and cellular survival. Reduced expression of Beclin 1 has been observed in various carcinoma cell lines, such as MCF7, compared to ubiquitous expression in normal tissues, underscoring Beclin 1′s role in suppressing cancer development. Additionally, Beclin 1 shares structural similarities with the yeast autophagy gene product Apg6, highlighting conserved function in autophagy across different organisms. In transfected cells, Beclin 1 not only complements autophagocytosis but also inhibits cellular proliferation, further emphasizing significance in regulating cell growth and maintaining cellular balance. Overall, Beclin 1 (G-11) antibody provides reliable and versatile detection of Beclin 1, making this tool essential for researchers investigating molecular mechanisms of autophagy, cancer biology, and related cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BECN1/Beclin-1 Antibody (G-11) References:

1. Beclin-1 and its role as a target for anticancer therapy.  |  Toton, E., et al. 2014. J Physiol Pharmacol. 65: 459-67. PMID: 25179078
2. The potential of Beclin 1 as a therapeutic target for the treatment of breast cancer.  |  Jung, YY., et al. 2016. Expert Opin Ther Targets. 20: 167-78. PMID: 26357854
3. New Insights Into Beclin-1: Evolution and Pan-Malignancy Inhibitor Activity.  |  Wechman, SL., et al. 2018. Adv Cancer Res. 137: 77-114. PMID: 29405978
4. Beclin-1 as a neutrophil-specific immune checkpoint.  |  Su, YL. and Kortylewski, M. 2019. J Clin Invest. 129: 5079-5081. PMID: 31657787
5. The Role of Beclin 1-Dependent Autophagy in Cancer.  |  Vega-Rubín-de-Celis, S. 2019. Biology (Basel). 9: PMID: 31877888
6. Autophagy-Related Beclin 1 and Head and Neck Cancers.  |  Hu, YJ., et al. 2020. Onco Targets Ther. 13: 6213-6227. PMID: 32669852
7. Regulation of Beclin 1-Mediated Autophagy by Oncogenic Tyrosine Kinases.  |  Vega-Rubín-de-Celis, S., et al. 2020. Int J Mol Sci. 21: PMID: 33287140
8. Beclin-1-Dependent Autophagy Improves Outcomes of Pneumonia-Induced Sepsis.  |  Nikouee, A., et al. 2021. Front Cell Infect Microbiol. 11: 706637. PMID: 34211859
9. Targeting autophagy and beyond: Deconvoluting the complexity of Beclin-1 from biological function to cancer therapy.  |  Ye, J., et al. 2023. Acta Pharm Sin B. 13: 4688-4714. PMID: 38045051
10. Beclin 1 Haploinsufficiency Ameliorates High-Fat Diet-Induced Myocardial Injury via Inhibiting Alternative Mitophagy.  |  Zeng, X., et al. 2024. Antioxid Redox Signal. 40: 906-925. PMID: 38251672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BECN1/Beclin-1 Antibody (G-11) has 70 citations in a variety of scientific publications.

Primary antibodies like Anti-BECN1/Beclin-1 Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BECN1/Beclin-1 Antibody (G-11) Specifications

Alternate Product Name: Beclin 1

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: Beclin 1

Epitope Species: human

Product Reactivity/Detection: Beclin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 70 citations in various scientific publications

Available Conjugates/Forms for BECN1/Beclin-1 Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred BECN1/Beclin-1 antibody is BECN1/Beclin-1 Antibody (E-8): sc-48341.