BCIP/NBT-Blue Liquid Substrate System for Membranes
See product citations (17)
Alternate Names:
5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system
Application:
BCIP®/NBT-Blue Liquid Substrate System for Membranes is a substrate for AP based detection systems including western blotting
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
BCIP®/NBT-Blue Liquid Substrate System for Membranes is a 5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate formulation used for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive. Recommended for use with Crystal Mounting Medium (sc-24943).
BCIP/NBT-Blue Liquid Substrate System for Membranes References
- Pea seed mitochondria are endowed with a remarkable tolerance to extreme physiological temperatures. | Stupnikova, I., et al. 2006. Plant Physiol. 140: 326-35. PMID: 16377742
- Proteolytic cleavage of HIV-1 GFP-Vpr fusions at novel sites within virions and living cells: concerns for intracellular trafficking studies. | Caly, L., et al. 2009. J Fluoresc. 19: 567-73. PMID: 19067127
- An acquired, calcium-dependent, factor X inhibitor. | Broze, GJ. 2014. Blood Cells Mol Dis. 52: 116-20. PMID: 24074948
- Increased expression of PELP1 in human sperm is correlated with decreased semen quality. | Skibinska, I., et al. 2018. Asian J Androl. 20: 425-431. PMID: 29676290
- Clickable cellulosic surfaces for peptide-based bioassays. | Odinolfi, MT., et al. 2019. Talanta. 205: 120152. PMID: 31450458
- A new histochemical stain for neutrophilic leukocyte alkaline phosphatase. | Wolf, PL., et al. 1968. Enzymologia. 35: 154-6. PMID: 4176788
- A test for bacterial alkaline phosphatase: use in rapid identification of Serratia organisms. | Wolf, PL., et al. 1973. Clin Chem. 19: 1248-9. PMID: 4758599
- Substrates for cytochemical demonstration of enzyme activity. II. Some dihalo-3-indolyl phosphates and sulfates. | Horwitz, JP., et al. 1966. J Med Chem. 9: 447. PMID: 5960940
- Generation of mouse hybridomas secreting anti-salmonella enteritidis antibodies and their preliminary characterization | Buyuktanir, O., Yagci, T., Findik, A., Yildirim, T., & Yurdusev, N. 2011. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 17(4): 667-670.
Substrate of:
Chromogenic, Enzyme, and HRP and AP.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCIP/NBT-Blue Liquid Substrate System for Membranes, 100 ml | sc-291929 | 100 ml | $168.00 |