Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BAF170 Double Nickase Plasmid (h): sc-402023-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BAF170 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BAF170 Double Nickase Plasmid (h) and BAF170 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SMARCC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BAF170 Antibody (E-6): sc-17838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAF170 Double Nickase Plasmid (h) | sc-402023-NIC | 20 µg | $410.00 | |||
BAF170 Double Nickase Plasmid (h2) | sc-402023-NIC-2 | 20 µg | $410.00 |
SMARCC2 encodes BAF170, a core structural subunit of the SWI/SNF (BAF) ATP-dependent chromatin remodeling complex that regulates nucleosome positioning and higher-order chromatin organization. BAF170 supports lineage-specific transcriptional programs by coordinating enhancer and promoter accessibility, thereby influencing processes such as neural development, cell-cycle control, and differentiation. Through interactions with factors in DNA damage response and transcriptional regulation pathways, SMARCC2 contributes to maintenance of genome stability and epigenetic state. Altered SWI/SNF complex composition or disruption of SMARCC2-associated chromatin remodeling has been linked to aberrant gene expression programs observed across multiple disease contexts, including cancer and neurodevelopmental disorders.
BAF170 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SMARCC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SMARCC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SMARCC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SMARCC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.