BAF155 Anticuerpo (DXD7) recomendado para detectar BAF155 de human y en menor medida, mouse, rat origen, mediante WB, IP, IF y IHC(P)

BAF155 Anticuerpo (DXD7): sc-32763

BAF155 Anticuerpo (DXD7) clasificado 4.2 de 5 por 10.
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Ficha técnica

    • Anti-BAF155 Anticuerpo DXD7 es un monoclonal de ratón IgG1 κ BAF155 Anticuerpo, ver las 17 publicaciones, proporcionado como 200 µg/ml
    • raised against a synthetic peptide corresponding to amino acids in the BAF155 sequence of human origin
    • BAF155 Anticuerpo (DXD7) es recomendado para detectar BAF155 de human y en menor medida, mouse, rat origen, mediante WB, IP, IF y IHC(P)
    • BAF155 Anticuerpo (DXD7) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • TransCruz reactivo, para ChIP (sc-32763 X, 200 µg/0.1 ml)
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de BAF155 (DXD7): sc-32763.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for BAF155 Antibody (DXD7) for WB and IHC(P) applications. These reagents are now offered in bundles with BAF155 Antibody (DXD7) (see ordering information below).

When should the TransCruz reagent sc-32763 X be used instead of the standard form sc-32763?

Preguntado por: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 3 de 5 por de Baf155 (DXD7) used for western blot application 2.4ug of antibody used, detects the right band at expected molecular weight, but non specific bands at 55kDa have been observed as well.
Fecha de publicación: 2018-03-07
Rated 4 de 5 por de Good for Western Blot! We got strong bands after O/N incubation with primary at the right size. We tested it in melanoma lines. There were also some unspecific bands but far from the Baf155 specific band.
Fecha de publicación: 2017-10-19
Rated 3 de 5 por de Didn't work well on Western blot Very weak signal on Western blot. Not sure if dilution used was not high enough. Started at 1:500 and got faint bands for a signal. Had to reincubate overnight, which is not a great workflow.
Fecha de publicación: 2017-09-18
Rated 4 de 5 por de Good for IP The product is successful in endogenous BAF155 immunoprecipitation
Fecha de publicación: 2016-12-07
Rated 4 de 5 por de Produced robust immunoperoxidase nuclear Produced robust immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Fecha de publicación: 2015-05-20
Rated 5 de 5 por de Published data for IF in with human embryonic Published data for IF in with human embryonic fibroblast (HE) cells -SCBT Publication Review
Fecha de publicación: 2015-05-11
Rated 5 de 5 por de Good WB publishable data gathered with mouse Good WB publishable data gathered with mouse colon mucosa and neoplastic tissues/ COS7 cells. -SCBT Publication Review
Fecha de publicación: 2015-04-27
Rated 5 de 5 por de Works well for WB with Jurkat and HeLa nuclear Works well for WB with Jurkat and HeLa nuclear extracts. -SCBT QC
Fecha de publicación: 2015-04-06
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