Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ATP5B Double Nickase Plasmid (h): sc-401009-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATP5B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATP5B Double Nickase Plasmid (h) and ATP5B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP5B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATP5B Antibody (E-1): sc-55597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP5B Double Nickase Plasmid (h) | sc-401009-NIC | 20 µg | $410.00 | |||
ATP5B Double Nickase Plasmid (h2) | sc-401009-NIC-2 | 20 µg | $410.00 |
ATP5B encodes the β subunit of mitochondrial ATP synthase (Complex V), a core catalytic component that couples proton flux across the inner mitochondrial membrane to ATP production via oxidative phosphorylation. By governing cellular energy charge, ATP5B influences mitochondrial membrane potential, redox balance, and ATP-dependent processes such as ion homeostasis, protein synthesis, and stress responses. Dysregulated ATP synthase activity and broader mitochondrial dysfunction are linked to altered bioenergetics and reactive oxygen species handling observed across diverse human diseases, including neuromuscular and neurodegenerative phenotypes. As a highly conserved mitochondrial gene product, ATP5B is frequently used to interrogate mitochondrial metabolism and its crosstalk with apoptosis and integrated stress signaling.
ATP5B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP5B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP5B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP5B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP5B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.