ASS1 Double Nickase Plasmid (h): sc-401923-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ASS1 Double Nickase Plasmid (h) and ASS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ASS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ASS1 Antibody (E-12): sc-365475
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASS1 Double Nickase Plasmid (h) | sc-401923-NIC | 20 µg | $410.00 | |||
ASS1 Double Nickase Plasmid (h2) | sc-401923-NIC-2 | 20 µg | $410.00 |
Human ASS1 (argininosuccinate synthase 1) catalyzes the ATP-dependent condensation of citrulline and aspartate to form argininosuccinate, a core step in the urea cycle that enables ammonia detoxification and supports systemic nitrogen homeostasis. Beyond hepatic ureagenesis, ASS1 activity influences intracellular arginine availability, linking urea cycle flux to nitric oxide biosynthesis and broader amino acid metabolism. Altered ASS1 expression or function is associated with urea cycle disorders such as citrullinemia type I and has been observed in tumor contexts where arginine auxotrophy and metabolic rewiring are under investigation. These features make ASS1 a useful node for studying nitrogen handling, mitochondrial–cytosolic metabolic coupling, and stress responses to amino acid limitation.
ASS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ASS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ASS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ASS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ASS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.