ASIC2 Double Nickase Plasmid (h): sc-403265-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASIC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ASIC2 Double Nickase Plasmid (h) and ASIC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ASIC2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASIC2 Double Nickase Plasmid (h) | sc-403265-NIC | 20 µg | $410.00 | |||
ASIC2 Double Nickase Plasmid (h2) | sc-403265-NIC-2 | 20 µg | $410.00 |
ASIC2 (acid-sensing ion channel subunit 2) encodes a proton-gated, amiloride-sensitive cation channel of the DEG/ENaC family that contributes to neuronal excitability and membrane depolarization in response to extracellular acidification. In human cells, ASIC2 can assemble with other ASIC subunits to tune ion selectivity, desensitization kinetics, and pH sensitivity, linking local pH shifts to Ca2+/Na+ influx and downstream signaling. ASIC2 activity intersects with processes such as synaptic transmission, mechanosensation, and inflammatory microenvironment sensing where extracellular pH fluctuates. Altered ASIC2 expression or channel function has been investigated in neurophysiology and pain-related signaling, and is relevant to research on neuronal stress responses and excitotoxic pathways.
ASIC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ASIC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ASIC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ASIC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ASIC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.