Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARRDC1 Double Nickase Plasmid (h): sc-407858-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARRDC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARRDC1 Double Nickase Plasmid (h) and ARRDC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARRDC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARRDC1 Antibody (E-6): sc-398652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARRDC1 Double Nickase Plasmid (h) | sc-407858-NIC | 20 µg | $410.00 |
Arrestin domain containing 1 (ARRDC1) is a plasma membrane–associated adaptor implicated in endosomal trafficking and ubiquitin-dependent regulation of membrane protein turnover. ARRDC1 recruits NEDD4 family E3 ubiquitin ligases to promote cargo ubiquitination and participates in the generation of ARRDC1-mediated microvesicles, linking it to extracellular vesicle biogenesis and intercellular communication. Through these functions, ARRDC1 influences receptor signaling dynamics, including pathways that depend on membrane receptor internalization and recycling. Dysregulated vesicle trafficking and ubiquitin signaling involving ARRDC1 has been explored in contexts such as cancer cell signaling and viral budding processes, making it relevant for mechanistic studies of membrane remodeling.
ARRDC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARRDC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARRDC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARRDC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARRDC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.