Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARID1B CRISPR/Cas9 KO Plasmid (m): sc-433726
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARID1B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARID1B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARID1B Antibody (KMN1): sc-32762
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARID1B CRISPR/Cas9 KO Plasmid (m) | sc-433726 | 20 µg | $397.00 |
Overview
Arid1b encodes ARID1B, an AT-rich interaction domain–containing subunit of the SWI/SNF (BAF) ATP-dependent chromatin remodeling complex that regulates nucleosome positioning and enhancer accessibility. Through control of chromatin state, ARID1B influences transcriptional programs governing cell fate specification, proliferation, and differentiation, with prominent roles in neural development and lineage-restricted gene expression. ARID1B participates in epigenetic regulation that interfaces with pathways such as Wnt/β-catenin, Notch, and cell-cycle control, shaping context-dependent transcriptional outputs. Disruption of ARID1B function has been linked to developmental disorders and neurodevelopmental phenotypes, and altered SWI/SNF composition is frequently studied in disease-relevant models of transcriptional dysregulation.
ARID1B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Arid1b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Arid1b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Arid1b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARID1B protein expression.
This CRISPR knockout system enables efficient generation of Arid1b-deficient cell models for investigation of ARID1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Arid1b exon(s) critical for ARID1B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Arid1b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARID1B CRISPR/Cas9 KO Plasmid (m) and ARID1B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Arid1b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARID1B HDR Plasmid (m) and ARID1B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Arid1b homology arms to support homology-directed repair at defined Arid1b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.