Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARA70 Double Nickase Plasmid (m): sc-424185-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARA70 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARA70 Double Nickase Plasmid (m) and ARA70 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ncoa4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARA70 Antibody (C-4): sc-373739
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARA70 Double Nickase Plasmid (m) | sc-424185-NIC | 20 µg | $410.00 |
Mouse Ncoa4 encodes ARA70, a nuclear receptor coactivator that modulates ligand-dependent transcription by interacting with androgen receptor and related nuclear receptor complexes. ARA70 influences chromatin-associated transcriptional programs that regulate cell proliferation, differentiation, and metabolism, integrating signals across steroid hormone and co-regulator pathways. NCOA4 also participates in iron homeostasis through ferritinophagy by partnering with NCOA4-directed autophagy machinery to deliver ferritin to lysosomes, linking nuclear receptor biology to autophagy and oxidative stress responses. Dysregulation of these processes has been associated with altered hormone signaling, iron-dependent cell vulnerability, and phenotypes relevant to cancer biology and neurodegeneration research contexts.
ARA70 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ncoa4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ncoa4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ncoa4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ncoa4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.