Aquaporin 9/AQP9 Double Nickase Plasmid (h): sc-401298-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 9/AQP9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aquaporin 9/AQP9 Double Nickase Plasmid (h) and Aquaporin 9/AQP9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AQP9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 9/AQP9 Antibody (G-3): sc-74409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 9/AQP9 Double Nickase Plasmid (h) | sc-401298-NIC | 20 µg | $410.00 | |||
Aquaporin 9/AQP9 Double Nickase Plasmid (h2) | sc-401298-NIC-2 | 20 µg | $410.00 |
AQP9 encodes aquaporin-9, a plasma membrane channel that facilitates transmembrane flux of water and small neutral solutes, including glycerol and other polyols, thereby linking osmotic balance to intermediary metabolism. In hepatocytes and immune cells, AQP9 contributes to glycerol uptake and carbon handling that can influence gluconeogenic and lipid metabolic processes, while also participating in cellular volume regulation. Its expression is regulated by inflammatory and metabolic cues, connecting AQP9 to pathways governing nutrient transport, stress responses, and cell-state transitions. Dysregulated AQP9 levels have been reported in contexts of metabolic dysfunction and inflammatory disease biology, making it a useful target for mechanistic studies of membrane transport and metabolic reprogramming.
Aquaporin 9/AQP9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AQP9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AQP9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AQP9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AQP9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.