APPL2 Antibody (F-2): sc-398859

APPL2 Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects APPL2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APPL2 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APPL2 plays a crucial role in cellular signaling and trafficking, particularly in epidermal growth factor (EGF) signaling pathways. APPL2 interacts with GTPase Rab 5, facilitating the translocation of signaling complexes from the plasma membrane to the nucleus, where APPL2 associates with chromatin remodeling complexes such as NuRD/MeCP1. This interaction is vital for regulating gene expression and promoting cell growth and proliferation. APPL2 is highly expressed in key tissues, including the heart, brain, skeletal muscle, and kidney, and shares 54% homology with APPL1, underscoring APPL2′s importance in cellular functions. Additionally, APPL2 has been shown to associate with the follicle-stimulating hormone receptor, further highlighting APPL2′s role in reproductive signaling pathways.

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APPL2 Antibody (F-2) References:

1. Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity.  |  Thomas, CC., et al. 2001. Biochem J. 358: 287-94. PMID: 11513726
2. The B cell SH2/PH domain-containing adaptor Bam32/DAPP1 is required for T cell-independent II antigen responses.  |  Fournier, E., et al. 2003. Curr Biol. 13: 1858-66. PMID: 14588241
3. The BAR-domain family of proteins: a case of bending and binding?  |  Habermann, B. 2004. EMBO Rep. 5: 250-5. PMID: 14993925
4. APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment.  |  Miaczynska, M., et al. 2004. Cell. 116: 445-56. PMID: 15016378
5. Human follicle-stimulating hormone (FSH) receptor interacts with the adaptor protein APPL1 in HEK 293 cells: potential involvement of the PI3K pathway in FSH signaling.  |  Nechamen, CA., et al. 2004. Biol Reprod. 71: 629-36. PMID: 15070827
6. Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1.  |  Lo, HW., et al. 2006. J Cell Biochem. 98: 1570-83. PMID: 16552725
7. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function.  |  Mao, X., et al. 2006. Nat Cell Biol. 8: 516-23. PMID: 16622416
8. APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction.  |  Lin, DC., et al. 2006. Mol Cell Biol. 26: 8928-41. PMID: 17000777
9. GIPC is recruited by APPL to peripheral TrkA endosomes and regulates TrkA trafficking and signaling.  |  Varsano, T., et al. 2006. Mol Cell Biol. 26: 8942-52. PMID: 17015470
10. APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex.  |  Nechamen, CA., et al. 2007. Mol Cell Endocrinol. 260-262: 93-9. PMID: 17030088
11. A conserved motif in the ITK PH-domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions.  |  Hirve, N., et al. 2012. PLoS One. 7: e45158. PMID: 23028816
12. Characterization and distribution of adaptor protein containing a PH domain, PTB domain and leucine zipper motif (APPL1) in Alzheimer's disease hippocampus: an immunohistochemical study.  |  Ogawa, A., et al. 2013. Brain Res. 1494: 118-24. PMID: 23246927

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-APPL2 Antibody (F-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-APPL2 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

APPL2 Antibody (F-2) Specifications

Alternate Product Name: APPL2

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 31-54

Epitope Target: APPL2

Epitope Species: human

Product Reactivity/Detection: APPL2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for APPL2 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin