Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
apoH CRISPR/Cas9 KO Plasmid (m): sc-419168
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apoH CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the apoH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: apoH Antibody (C-2): sc-515677
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apoH CRISPR/Cas9 KO Plasmid (m) | sc-419168 | 20 µg | $397.00 |
Overview
Apoh encodes apolipoprotein H (apoH; β2-glycoprotein I), a circulating phospholipid-binding glycoprotein that associates with anionic membranes and lipoprotein particles. In mice, apoH participates in lipid transport and clearance processes and interfaces with coagulation-related and complement-associated pathways through its interactions with negatively charged phospholipids exposed during cellular activation or damage. Altered APOH/apoH biology is linked to dysregulated hemostasis and inflammatory vascular processes, and it is frequently studied in the context of autoantibody-mediated phospholipid recognition. These functions make Apoh a useful target for investigating lipid–immune crosstalk, membrane-binding protein biology, and mechanisms that connect inflammation to thrombosis-related phenotypes in experimental models.
apoH CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Apoh gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Apoh together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Apoh open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish apoH protein expression.
This CRISPR knockout system enables efficient generation of Apoh-deficient cell models for investigation of apoH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Apoh exon(s) critical for apoH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Apoh genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by apoH CRISPR/Cas9 KO Plasmid (m) and apoH CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Apoh locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by apoH HDR Plasmid (m) and apoH HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Apoh homology arms to support homology-directed repair at defined Apoh target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.