apoB Double Nickase Plasmid (h): sc-400705-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apoB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- apoB Double Nickase Plasmid (h) and apoB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APOB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: apoB Antibody (A-6): sc-393636
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apoB Double Nickase Plasmid (h) | sc-400705-NIC | 20 µg | $410.00 | |||
apoB Double Nickase Plasmid (h2) | sc-400705-NIC-2 | 20 µg | $410.00 |
APOB encodes apolipoprotein B (apoB), an essential structural component of atherogenic lipoproteins that scaffolds very low-density lipoprotein (VLDL) assembly in hepatocytes and is processed to apoB-100 for circulation. ApoB coordinates lipidation and secretion of triglyceride- and cholesterol-rich particles through pathways involving microsomal triglyceride transfer protein (MTP) and the endoplasmic reticulum secretory program. In peripheral tissues, apoB-containing lipoproteins are central to LDL receptor–mediated uptake, coupling lipid transport to cellular cholesterol homeostasis and downstream signaling. Perturbation of APOB expression or apoB particle production is linked to dyslipidemia and lipid-driven vascular pathology, making it a core target for mechanistic studies of lipoprotein metabolism.
apoB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APOB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APOB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APOB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APOB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.