alpha-Actin Antibody (SPM332): sc-56499

α-Actin Antibody (SPM332) is a mouse monoclonal IgG2a kappa light chain antibody that detects smooth muscle α-Actin from mouse, rat, human, and bovine sources through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). α-Actin Antibody (SPM332) comes in a non-conjugated form for flexible research applications. Actin maintains cell shape, enables motility, and facilitates intracellular transport. α-Actin exists in a dynamic balance between monomeric globular actin (G-actin) and filamentous actin (F-actin), allowing quick assembly and disassembly in response to cellular signals. This adaptability is crucial for muscle contraction and cell division, where precise actin regulation ensures proper function. α-Actin is predominantly expressed in muscle cells, unlike β-Actin and γ-Actin found in non-muscle tissues. Small GTPases and other signaling pathways regulate α-Actin organization in the cytoskeleton, highlighting α-Actin′s significance in muscle function and cellular processes.

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alpha-Actin Antibody (SPM332) References:

1. Myocardin and Prx1 contribute to angiotensin II-induced expression of smooth muscle alpha-actin.  |  Yoshida, T., et al. 2004. Circ Res. 94: 1075-82. PMID: 15016729
2. The vinculin/sarcomeric-alpha-actinin/alpha-actin nexus in cultured cardiac myocytes.  |  Lu, MH., et al. 1992. J Cell Biol. 117: 1007-22. PMID: 1577864
3. Force activates smooth muscle alpha-actin promoter activity through the Rho signaling pathway.  |  Zhao, XH., et al. 2007. J Cell Sci. 120: 1801-9. PMID: 17456553
4. Cell-specific activation of the human skeletal alpha-actin by androgens.  |  Hong, MH., et al. 2008. Endocrinology. 149: 1103-12. PMID: 18063690
5. PIAS1 mediates TGFbeta-induced SM alpha-actin gene expression through inhibition of KLF4 function-expression by protein sumoylation.  |  Kawai-Kowase, K., et al. 2009. Arterioscler Thromb Vasc Biol. 29: 99-106. PMID: 18927467
6. Smooth muscle α actin is specifically required for the maintenance of lactation.  |  Weymouth, N., et al. 2012. Dev Biol. 363: 1-14. PMID: 22123032
7. The Early-Onset Myocardial Infarction Associated PHACTR1 Gene Regulates Skeletal and Cardiac Alpha-Actin Gene Expression.  |  Kelloniemi, A., et al. 2015. PLoS One. 10: e0130502. PMID: 26098115
8. Prevalence and phenotypes of congenital myopathy due to α-actin 1 gene mutations.  |  Witting, N., et al. 2016. Muscle Nerve. 53: 388-93. PMID: 26172852
9. Description of a Novel Cardiac Phenotype Associated With a Missense Variant in the Cardiac α-Actin (ACTC1) Gene.  |  Toledano-Delgado, FJ., et al. 2023. Circ Genom Precis Med. 16: e003963. PMID: 36960733
10. Circulating alpha-actin in non-insulin-dependent diabetics with autonomic dysfunction.  |  Prados, J., et al. 1995. Int J Cardiol. 51: 127-30. PMID: 8522407

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-Actin Antibody (SPM332) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-α-Actin Antibody (SPM332) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-Actin Antibody (SPM332) Specifications

Alternate Product Name: ACTA2

Product Clone Name: SPM332

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-10

Epitope Target: α-Actin

Epitope Species: human

Product Reactivity/Detection: smooth muscle α-Actin

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for α-Actin Antibody (SPM332): N/A

The preferred α-Actin antibody is α-Actin Antibody (1A4): sc-32251.