Alkaline Phosphatase Anticuerpo (A-10) recomendado para detectar PLAP, ALPPL2 and IAP of human origin, and Akp-3 of mouse and rat origin medainte WB, IP, IF, IHC(P) y ELISA

Alkaline Phosphatase Anticuerpo (A-10): sc-271431

Alkaline Phosphatase Anticuerpo (A-10) clasificado 3.8 de 5 por 5.
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Ficha técnica

    • Anti-Alkaline Phosphatase Anticuerpo A-10 es un monoclonal de ratón IgG2a κ Alkaline Phosphatase Anticuerpo, ver las 33 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 66-111 de la región interna de PLAP de origen human
    • Alkaline Phosphatase Anticuerpo (A-10) es recomendado para detectar PLAP, ALPPL2 and IAP of human origin, and Akp-3 of mouse and rat origin medainte WB, IP, IF, IHC(P) y ELISA
    • Alkaline Phosphatase Anticuerpo (A-10) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Alkaline Phosphatase (A-10): sc-271431.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Alkaline Phosphatase Antibody (A-10). This work is in progress.
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this antibody can be use in rat?

Preguntado por: tx3359836
Thank you for your question. Yes, this mouse monoclonal antibody, Alkaline Phosphatase (A-10): sc-271431, can be used in rat. We are aware of two publications citing use of the antibody in rat samples. These include PMID numbers: 25662160 and 26781793.
Respondida por: Technical Support
Fecha de publicación: 2018-11-11

What application is the blocking peptide sc-271431 P appropriate for?

Preguntado por: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Used in Mesenchymal Stem Cells This antibody worked well with MSCs, used 1:500 dilution.
Fecha de publicación: 2019-09-12
Rated 3 de 5 por de Does not detect SEAP in western blot I tried to detect exogenously expressed SEAP (secreted alkaline phosphatase - a truncated version of PLAP) in HeLa cell lysate and media supernatant with this antibody in western blot (1:200 dilution). It didn't detect SEAP either in media or in cell lysate, even if the immunogen of this antibody should also be present in SEAP.
Fecha de publicación: 2018-02-23
Rated 1 de 5 por de A Blur of False Hope and Empty Promises Used a 1:250 dilution attempting to probe human osteosarcoma cells. The result was a faint blur at approximately the correct size, but by no means a well-define band. Attempted to incubate primary antibody again at 4 degrees overnight, and the band disappeared. No concentration of primary or secondary antibodies could resurrect it.
Fecha de publicación: 2018-01-05
Rated 5 de 5 por de IHC/P Work well in the IHC/P of mouse tibia(1:100). Osteoblasts and other cells could be stained.
Fecha de publicación: 2017-10-23
Rated 5 de 5 por de Great Western blot data of PLAP expression in non Great Western blot data of PLAP expression in non-transfected and human PLAP transfected 293T whole cell lysates. -SCBT QC
Fecha de publicación: 2013-12-01
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