Aldolase C Anticuerpo (H-11) recomendado para detectarAldolase C de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

Aldolase C Anticuerpo (H-11): sc-271593

Aldolase C Anticuerpo (H-11) clasificado 3.6 de 5 por 5.
  • y_2021, m_9, d_25, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_es_ES, sid_271593, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 123ms
  • REVIEWS, PRODUCT
loading
5
1
4
3
3
0
2
0
1
1

Ficha técnica

    • Anti-Aldolase C Anticuerpo H-11 es un monoclonal de ratón IgG1 κ Aldolase C Anticuerpo, ver las 7 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 77-112 de la región interna de Aldolase C de origen human
    • Aldolase C Anticuerpo (H-11) es recomendado para detectar Aldolase C de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; también reactivo con otras especies, incluyendo anyd equine, canine, bovine and porcine
    • Aldolase C Anticuerpo (H-11) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Aldolase C (H-11): sc-271593.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Aldolase C Antibody (H-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Aldolase C Antibody (H-11) (see ordering information below).
loading

请问该抗体用于组织的免疫荧光染色是否需要先用Triton X-100处理?是否可以给一些建议?谢谢

Preguntado por: quew98230
'Is treatment with Triton X-100 needed when using this antibody for immunofluorescence cell staining?' Thank you for your question. You can find our recommended IF protocol on our website as Protocol 5: Immunofluorescence Cell Staining. If fixing in formalin, we recommend you include a cell permeabilization treatment. After fixing the cells, wash in three changes of PBS. Then incubate for 3 minutes in a solution of 0.01% Triton X-100 in PBS. If you have further questions, feel free to contact our technical service directly.
Respondida por: Technical Support Europe
Fecha de publicación: 2019-04-03

Is this antibody very specific to Aldolase C? Can it detect other isoforms of aldolase, such as aldolase A (I want to distinguish these two isoforms)?

Preguntado por: Lin WANG
Thank you for your question. This antibody, sc-271593, is specific for Aldolase C. Please contact Technical Service if you have any further questions.
Respondida por: Tech Service
Fecha de publicación: 2019-01-14

What application is the blocking peptide sc-271593 P appropriate for?

Preguntado por: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
  • y_2021, m_9, d_25, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_es_ES, sid_271593, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 125ms
  • QUESTIONS, PRODUCT
Rated 1 de 5 por de Does not work well The new batch of Aldolase C antibody (H11) gives very high background when used at prescribed 1:200 dilution. It does not work at 1:500 dilution. It does not work as well as it should. We should ask about a fresh batch of the antibody.
Fecha de publicación: 2020-08-10
Rated 4 de 5 por de 很好用 B细胞,稀释比例1:500,条带清晰,与预测位置一致。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。
Fecha de publicación: 2017-10-10
Rated 5 de 5 por de work well This antibody worked well for both Western Blotting in mouse spinal cord and the dilution of this is 1:1000
Fecha de publicación: 2016-12-23
Rated 4 de 5 por de Bands seen in WB using mouse brain and human Bands seen in WB using mouse brain and human hippocampus tissue extracts. -SCBT QC
Fecha de publicación: 2015-06-29
Rated 4 de 5 por de Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Fecha de publicación: 2013-12-17
  • y_2021, m_9, d_25, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_es_ES, sid_271593, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT