Date published: 2026-4-27
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AK2 Antibody (F-2) - Western Blotting - Image 365497
AK2 Antibody (F-2): sc-374095. Western blot analysis of AK2 expression in TF-1 (A), M1 (B), KNRK (C) and c4 (D) whole cell lysates.
AK2 Antibody (F-2): sc-374095. Western blot analysis of AK2 expression in TF-1 (A), M1 (B), KNRK (C) and c4 (D) whole cell lysates.

AK2 Antibody (F-2): sc-374095

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Datasheets
  • AK2 Antibody (F-2) is a mouse monoclonal IgG1 κ AK2 antibody, cited in 5 publications, provided at 200 µg/ml
  • raised against amino acids 31-95 mapping near the N-terminus of AK2 of human origin
  • AK2 Antibody (F-2) is recommended for detection of AK2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-AK2 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AK2 Antibody (F-2) for WB and IHC(P) applications. These reagents are now offered in bundles with AK2 Antibody (F-2) (see ordering information below).
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Additional Adenylate Kinase Antibodies including AK1, AK2, AK3 and AK4

AK2 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AK2 Antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AK2 plays a crucial role in cellular energy metabolism through involvement in adenine nucleotide phosphorylation, essential for energy transfer within cells. AK2 (F-2) antibody targets proteins localized in mitochondrial intermembrane space, where AK2 participates in ATP level regulation, influencing metabolic pathways and cellular signaling. This specific localization enables rapid response to changes in cellular energy demands, especially during stress conditions such as apoptosis, where AK2 translocates to the cytosol alongside cytochrome c. AK2′s movement from mitochondria to cytosol during early apoptotic events suggests a role in cell death pathways and highlights mitochondrial function in maintaining cellular homeostasis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

AK2 Antibody (F-2) References:

  1. Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis.  |  Köhler, C., et al. 1999. FEBS Lett. 447: 10-2. PMID: 10218571
  2. Adenylate kinase phosphotransfer communicates cellular energetic signals to ATP-sensitive potassium channels.  |  Carrasco, AJ., et al. 2001. Proc Natl Acad Sci U S A. 98: 7623-8. PMID: 11390963
  3. Adenylate kinase 2, a mitochondrial enzyme.  |  Bruns, GA. and Regina, VM. 1977. Biochem Genet. 15: 477-86. PMID: 195572
  4. Cloning and characterization of cDNA for human adenylate kinase 2A.  |  Lee, Y., et al. 1996. Biochem Mol Biol Int. 39: 833-42. PMID: 8843353
  5. cDNA cloning and tissue-specific expression of the gene encoding human adenylate kinase isozyme 2.  |  Noma, T., et al. 1998. Biochim Biophys Acta. 1395: 34-9. PMID: 9434148
  6. Cloning and expression of human adenylate kinase 2 isozymes: differential expression of adenylate kinase 1 and 2 in human muscle tissues.  |  Lee, Y., et al. 1998. J Biochem. 123: 47-54. PMID: 9504408
  7. Adenylate kinase: kinetic behavior in intact cells indicates it is integral to multiple cellular processes.  |  Dzeja, PP., et al. 1998. Mol Cell Biochem. 184: 169-82. PMID: 9746320

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

AK2 Antibody (F-2)

sc-374095
200 µg/ml
$322.00

AK2 Antibody (F-2): m-IgG Fc BP-HRP Bundle

sc-529658
200 µg Ab; 10 µg BP
$361.00

AK2 Antibody (F-2): m-IgGκ BP-HRP Bundle

sc-522619
200 µg Ab, 40 µg BP
$361.00

AK2 Antibody (F-2): m-IgG1 BP-HRP Bundle

sc-543626
200 µg Ab; 20 µg BP
$361.00

AK2 Antibody (F-2) AC

sc-374095 AC
500 µg/ml, 25% agarose
$424.00

AK2 Antibody (F-2) HRP

sc-374095 HRP
200 µg/ml
$322.00

AK2 Antibody (F-2) FITC

sc-374095 FITC
200 µg/ml
$336.00

AK2 Antibody (F-2) PE

sc-374095 PE
200 µg/ml
$349.00

AK2 Antibody (F-2) Alexa Fluor® 488

sc-374095 AF488
200 µg/ml
$364.00

AK2 Antibody (F-2) Alexa Fluor® 546

sc-374095 AF546
200 µg/ml
$364.00

AK2 Antibody (F-2) Alexa Fluor® 594

sc-374095 AF594
200 µg/ml
$364.00

AK2 Antibody (F-2) Alexa Fluor® 647

sc-374095 AF647
200 µg/ml
$364.00

AK2 Antibody (F-2) Alexa Fluor® 680

sc-374095 AF680
200 µg/ml
$364.00

AK2 Antibody (F-2) Alexa Fluor® 790

sc-374095 AF790
200 µg/ml
$364.00

I am using AK2 (F-2): sc-374095 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Nice band at expected molecular weightTested in WB on mouse purified mitochondria and there was a nice band at the expected MW + an aspecific band at about 97 KDa
Date published: 2018-01-17
Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Highly potent and specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~25 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-10
Rated 5 out of 5 by from Satisfactory Western Blot data of AK2 expressionSatisfactory Western Blot data of AK2 expression in HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2014-12-01
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AK2 Antibody (F-2) is rated 5.0 out of 5 by 5.
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