AIP5 Double Nickase Plasmid (h): sc-403772-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AIP5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AIP5 Double Nickase Plasmid (h) and AIP5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WWP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AIP5 Antibody (F-3): sc-390897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AIP5 Double Nickase Plasmid (h) | sc-403772-NIC | 20 µg | $410.00 | |||
AIP5 Double Nickase Plasmid (h2) | sc-403772-NIC-2 | 20 µg | $410.00 |
WWP1 encodes the human E3 ubiquitin-protein ligase AIP5, a HECT-domain enzyme that ubiquitinates diverse substrates to regulate protein stability, trafficking, and signal amplitude. Through PPxY motif recognition via its WW domains, AIP5 helps tune pathways controlling cell growth, polarity, and stress responses, including modulation of receptor-proximal signaling and turnover of regulatory proteins. Dysregulated WWP1/AIP5 activity has been associated with altered ubiquitin homeostasis and aberrant signaling programs implicated in oncogenic transformation and developmental phenotypes. Consequently, WWP1 is frequently studied to dissect how ubiquitin-mediated proteostasis interfaces with proliferation, migration, and pathway rewiring in human cells.
AIP5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WWP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WWP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WWP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WWP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.