AdSS2 CRISPR/Cas9 KO Plasmid (m): sc-419031
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AdSS2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AdSS2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AdSS2 CRISPR/Cas9 KO Plasmid (m) | sc-419031 | 20 µg | $397.00 |
Overview
Adss encodes adenylosuccinate synthase 2 (AdSS2), a key enzyme in de novo purine biosynthesis that catalyzes conversion of IMP to adenylosuccinate, supporting AMP production and cellular nucleotide balance. By controlling adenine nucleotide availability, AdSS2 influences DNA/RNA synthesis, energy metabolism, and proliferative capacity, and intersects with metabolic programs that couple purine flux to cell-cycle progression. Perturbation of purine biosynthetic enzymes is linked to altered growth phenotypes and stress responses, making AdSS2 relevant to studies of metabolic reprogramming in cancer biology and other contexts where nucleotide demand is elevated. In mouse models, Adss provides a tractable node for dissecting how purine supply constrains biosynthesis and signaling pathways dependent on adenine nucleotides.
AdSS2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adss gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adss together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adss open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AdSS2 protein expression.
This CRISPR knockout system enables efficient generation of Adss-deficient cell models for investigation of AdSS2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adss exon(s) critical for AdSS2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adss genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AdSS2 CRISPR/Cas9 KO Plasmid (m) and AdSS2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adss locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AdSS2 HDR Plasmid (m) and AdSS2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adss homology arms to support homology-directed repair at defined Adss target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.