Adenylate cyclase 10/ADCY10 CRISPR/Cas9 KO Plasmid (h): sc-406235

Overview

ADCY10 encodes adenylate cyclase 10 (soluble adenylyl cyclase, sAC), an intracellular enzyme that generates cyclic AMP in response to bicarbonate and calcium signals rather than G protein–coupled receptor activation. By shaping cAMP microdomains, ADCY10 modulates PKA and EPAC signaling, influencing processes such as mitochondrial function, ion transport, sperm capacitation, and pH-sensing responses. sAC-derived cAMP integrates metabolic and acid–base cues with phosphorylation networks, affecting transcriptional programs and cellular stress adaptation. Dysregulated ADCY10 activity has been investigated in contexts including male infertility, renal and airway bicarbonate handling, and tumor-associated cAMP signaling rewiring, supporting its relevance for mechanistic pathway studies.

Adenylate cyclase 10/ADCY10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADCY10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADCY10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADCY10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Adenylate cyclase 10/ADCY10 protein expression.

This CRISPR knockout system enables efficient generation of ADCY10-deficient cell models for investigation of Adenylate cyclase 10/ADCY10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ADCY10 exon(s) critical for Adenylate cyclase 10/ADCY10 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ADCY10 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Adenylate cyclase 10/ADCY10 CRISPR/Cas9 KO Plasmid (h) and Adenylate cyclase 10/ADCY10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADCY10 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Adenylate cyclase 10/ADCY10 HDR Plasmid (h) and Adenylate cyclase 10/ADCY10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADCY10 homology arms to support homology-directed repair at defined ADCY10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.