ADAR1 Double Nickase Plasmid (m): sc-425147-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAR1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADAR1 Double Nickase Plasmid (m) and ADAR1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Adar. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADAR1 Antibody (15.8.6): sc-73408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAR1 Double Nickase Plasmid (m) | sc-425147-NIC | 20 µg | $410.00 | |||
ADAR1 Double Nickase Plasmid (m2) | sc-425147-NIC-2 | 20 µg | $410.00 |
Mouse Adar encodes ADAR1, an adenosine deaminase acting on RNA that catalyzes A-to-I editing within double-stranded RNA structures, reshaping transcript sequences, splicing patterns, and RNA stability. ADAR1 is a central modulator of innate immune homeostasis by limiting aberrant recognition of endogenous dsRNA and influencing interferon-stimulated gene programs and RNA sensing pathways such as MDA5/MAVS. Through these functions it contributes to post-transcriptional regulation, stress responses, and control of immunogenic nucleic acids, making it relevant to studies of neurodevelopment, inflammation-associated phenotypes, and tumor immune signaling. Altered ADAR1 activity and RNA editing landscapes are widely used as molecular readouts for dysregulated RNA metabolism and immune activation in experimental models.
ADAR1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Adar locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Adar. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Adar function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Adar-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.