Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACTR-IIA CRISPR/Cas9 KO Plasmid (m): sc-418976
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACTR-IIA CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACTR-IIA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACTR-IIA Antibody (D-9): sc-515826
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACTR-IIA CRISPR/Cas9 KO Plasmid (m) | sc-418976 | 20 µg | $397.00 |
Overview
Acvr2a encodes activin A receptor type IIA (ACTR-IIA), a transmembrane serine/threonine kinase receptor that binds activins and related TGF-β superfamily ligands to regulate SMAD2/3-dependent transcription. In mouse cells, ACTR-IIA signaling influences embryonic patterning, reproductive axis regulation, and tissue homeostasis by coordinating differentiation, proliferation, and extracellular matrix programs. This pathway interfaces with other TGF-β family receptors and modulators to tune context-specific responses in development and remodeling. Dysregulated activin/ACTR-IIA signaling has been implicated in fibrotic remodeling, inflammation-associated tissue changes, and altered growth control, making it relevant for mechanistic studies of disease-associated phenotypes.
ACTR-IIA CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Acvr2a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Acvr2a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Acvr2a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACTR-IIA protein expression.
This CRISPR knockout system enables efficient generation of Acvr2a-deficient cell models for investigation of ACTR-IIA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Acvr2a exon(s) critical for ACTR-IIA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Acvr2a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACTR-IIA CRISPR/Cas9 KO Plasmid (m) and ACTR-IIA CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Acvr2a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACTR-IIA HDR Plasmid (m) and ACTR-IIA HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Acvr2a homology arms to support homology-directed repair at defined Acvr2a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.