Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACK Double Nickase Plasmid (h): sc-401482-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACK Double Nickase Plasmid (h) and ACK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACK Antibody (A-11): sc-28336
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACK Double Nickase Plasmid (h) | sc-401482-NIC | 20 µg | $410.00 | |||
ACK Double Nickase Plasmid (h2) | sc-401482-NIC-2 | 20 µg | $410.00 |
TNK2 encodes activated Cdc42-associated kinase 1 (ACK), a non-receptor tyrosine kinase that functions as a signaling hub downstream of receptor tyrosine kinases and small GTPases, including Cdc42. ACK modulates endocytosis and receptor trafficking through interactions with clathrin and adaptor proteins, and contributes to cytoskeletal dynamics, cell adhesion, and migration. It interfaces with pathways such as EGFR/RTK signaling, PI3K–AKT, and MAPK cascades, shaping cellular responses to growth-factor stimulation. Dysregulated TNK2/ACK activity and altered expression have been reported in cancer-associated signaling contexts and other diseases linked to aberrant RTK trafficking and kinase network remodeling.
ACK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.