Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACADVL (VLCAD) CRISPR/Cas9 KO Plasmid (m): sc-418939
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACADVL CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACADVL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACADVL Antibody (H-7): sc-376239
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACADVL (VLCAD) CRISPR/Cas9 KO Plasmid (m) | sc-418939 | 20 µg | $397.00 |
Overview
Acadvl encodes very long-chain acyl-CoA dehydrogenase (ACADVL/VLCAD), a mitochondrial flavoprotein that catalyzes the first dehydrogenation step of β-oxidation for long-chain fatty acyl-CoAs. This activity feeds reducing equivalents to the electron transfer flavoprotein system and supports oxidative energy production, particularly in tissues with high mitochondrial demand such as heart, skeletal muscle, and liver. Disruption of ACADVL perturbs lipid catabolism, promoting accumulation of long-chain acylcarnitines and shifting cellular metabolism toward compensatory glucose utilization and stress responses. As a core enzyme in fatty acid oxidation and mitochondrial homeostasis, Acadvl is widely studied in models of metabolic inflexibility, mitochondrial dysfunction, and lipid-driven cellular toxicity relevant to inborn errors of metabolism research.
ACADVL CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Acadvl gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Acadvl together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Acadvl open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACADVL protein expression.
This CRISPR knockout system enables efficient generation of Acadvl-deficient cell models for investigation of ACADVL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Acadvl exon(s) critical for ACADVL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Acadvl genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACADVL CRISPR/Cas9 KO Plasmid (m) and ACADVL CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Acadvl locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACADVL HDR Plasmid (m) and ACADVL HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Acadvl homology arms to support homology-directed repair at defined Acadvl target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.