ABHD3 Double Nickase Plasmid (h): sc-414947-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABHD3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABHD3 Double Nickase Plasmid (h) and ABHD3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABHD3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABHD3 Double Nickase Plasmid (h) | sc-414947-NIC | 20 µg | $410.00 |
Human ABHD3 (abhydrolase domain containing 3) is a serine hydrolase that contributes to lipid catabolism by hydrolyzing oxidized and medium-chain phospholipids, including lysophospholipid substrates, thereby influencing membrane lipid remodeling and lipid-derived signaling. Through regulation of phospholipid homeostasis, ABHD3 interfaces with pathways governing oxidative stress responses, inflammatory signaling, and organelle membrane dynamics. Altered ABHD3 expression or activity has been reported across contexts of metabolic dysregulation and tumor biology, where shifts in phospholipid composition can affect proliferation, migration, and cellular stress tolerance. As a result, ABHD3 is frequently studied in systems-level analyses of lipid metabolism, redox biology, and membrane-associated signaling networks.
ABHD3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABHD3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABHD3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABHD3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABHD3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.