ABC1 siRNA (h), shRNA y Partículas Lentivirales como silenciadores génicos human ABC1- siRNA específicos, Plásmido shRNA y Partículas Lentivirales como silenciadores génicos

ABC1 siRNA (h), shRNA y Partículas Lentivirales como silenciadores génicos

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Ficha técnica

    • Target species: human; for corresponding mouse product, see ABC1 siRNA and shRNA Plasmids (m)
    • Los silenciadores génicos, en general, consisten en un conjunto de cinco sequencias con dianas especificas, con una longitud de 19-25 nt
    • Para una verificación independiente de los resultados obtenidos con ABC1, podemos ofrecerles los plásmidos por separado, tras su petición
    • Anticuerpo Control Adecuado: ABC1 Antibody (AC10): sc-53482 está recomendado como control de la expresión del knockdown producido por ABC1 mediante Western Blot e Inmunofluorescencia
    • Para obtener más detalles sobre los Silenciadores Génicos si/shRNA haga click aquí
    • Los silenciadores génicos no están destinados al uso comercial, sólo para uso en investigación haga click aquí

Información del gen

EspeciesNombre del GenGen IDUbicación en el cromosomaNumero de Acceso de Isoforma (ARNm)Número de Acceso de la ProteínaNúmero OMIM™
HumanABCA1199q31.1XM_005251773, XM_005251776, XM_011518339, XM_011518340, XM_011518341, XM_011518342, XM_011518344, XM_017014378, XM_017014379, XM_017014380, XM_017014381, XM_017014382, NM_005502O95477604091

Does all sc lentivirus have the same promoters? The reason I ask is that I have seen others cited using sc lentivirus successfully in Thp-1 cells for LPL. I heard that promoter selection is crucial for Thp-1 cells.

Preguntado por: weng
Thank you for your question. Yes, our vector uses an H1 promoter for shRNA transcription.
Respondida por: Technical Support
Fecha de publicación: 2019-04-23

I read the reference cited here. Is the shRNA targeting the same sequence? I want to make a stable knockdown of ABCA1 in Thp-1 cells. Does your shRNA guarantee knockdown? Does the product come with a positive control for transfection efficiency?

Preguntado por: weng
Thank you for your question. Yes, the ABC1 shRNA plasmid (h): sc-41136-SH is using the same target sequence as the siRNA product that is referenced in the product citation on our website. For stable knockdown, you may want to consider using the shRNA Lentiviral Particles. Please contact our Technical Service Department for more information. We do sell separately a positive control for measuring transfection efficiency, copGFP Control Plasmid: sc-108083. https://www.scbt.com/scbt/browse/shrna-plasmid-support-products/_/N-lhyfam Santa Cruz does warranty these si/shRNA reagents to show observable knockdown at the protein level.  Santa Cruz implements computationally designed published algorithms which are demonstrated to give greater than 50% knockdown more than 90% of the time. If you purchase the reagents and are not able to achieve the expected results we encourage you to contact our technical support staff.  If we are unable to trouble-shoot the assay further then we are able to offer you either research credit or refund the purchase price of the reagents.
Respondida por: Technical Support
Fecha de publicación: 2019-04-23
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