ABAT Double Nickase Plasmid (h): sc-404041-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABAT Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABAT Double Nickase Plasmid (h) and ABAT Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABAT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ABAT Antibody (B-12): sc-393769
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABAT Double Nickase Plasmid (h) | sc-404041-NIC | 20 µg | $410.00 | |||
ABAT Double Nickase Plasmid (h2) | sc-404041-NIC-2 | 20 µg | $410.00 |
Human ABAT encodes 4-aminobutyrate aminotransferase (GABA transaminase), a mitochondrial pyridoxal phosphate–dependent enzyme that catalyzes GABA catabolism to succinic semialdehyde, linking neurotransmitter turnover to the tricarboxylic acid cycle via the GABA shunt. By regulating intracellular GABA levels and downstream redox and metabolic flux, ABAT influences neuronal excitability, mitochondrial homeostasis, and cellular energy balance. Perturbation of ABAT activity is associated with altered inhibitory neurotransmission and metabolic stress, and ABAT has been studied in the context of neurodevelopmental and seizure-related phenotypes as well as broader mitochondrial dysfunction. These features make ABAT a useful target for investigating GABAergic metabolism, mitochondrial pathways, and genotype–phenotype relationships in human cell models.
ABAT Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABAT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABAT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABAT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABAT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.