A33 Antibody (E-8): sc-398702

A33 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects A33 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). A33 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. A33 antigen is a transmembrane protein predominantly expressed in the intestinal epithelium and is found in 95% of human colon cancers, making A33 a significant marker for cancer research and potential therapeutic applications. A33 protein is located on the basolateral surfaces of intestinal epithelial cells across all lineages, which is crucial for maintaining intestinal barrier integrity and facilitating cell signaling. A33 antigen includes an extracellular domain with two immunoglobulin-like domains, a single-span transmembrane domain, and a highly acidic cytoplasmic domain, which is essential for cell adhesion and communication. A33 expression is regulated by the intestine-specific homeobox transcription factor CDX1 and the gut-enriched Kruppel-like factor (GKLF), with mutations in the GKLF binding sequence leading to reduced A33 antigen expression. Humanized anti-A33 antibody administration to colon cancer patients has shown promising results, highlighting A33′s importance in targeted cancer therapies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

A33 Antibody (E-8) References:

1. Organ-specific expression of the intestinal epithelium-related antigen A33, a cell surface target for antibody-based imaging and treatment in gastrointestinal cancer.  |  Sakamoto, J., et al. 2000. Cancer Chemother Pharmacol. 46 Suppl: S27-32. PMID: 10950144
2. Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells.  |  Johnstone, CN., et al. 2000. Am J Physiol Gastrointest Liver Physiol. 279: G500-10. PMID: 10960348
3. The murine A33 antigen is expressed at two distinct sites during development, the ICM of the blastocyst and the intestinal epithelium.  |  Abud, HE., et al. 2000. Mech Dev. 98: 111-4. PMID: 11044613
4. Analysis of the regulation of the A33 antigen gene reveals intestine-specific mechanisms of gene expression.  |  Johnstone, CN., et al. 2002. J Biol Chem. 277: 34531-9. PMID: 12114523
5. Transcriptional regulation of A33 antigen expression by gut-enriched Krüppel-like factor.  |  Mao, Z., et al. 2003. Oncogene. 22: 4434-43. PMID: 12853980
6. A33 antigen displays persistent surface expression.  |  Ackerman, ME., et al. 2008. Cancer Immunol Immunother. 57: 1017-27. PMID: 18236042
7. A33 antigen-deficient mice have defective colonic mucosal repair.  |  Pereira-Fantini, PM., et al. 2010. Inflamm Bowel Dis. 16: 604-12. PMID: 19856415
8. A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma.  |  Wong, NACS., et al. 2017. Histopathology. 71: 34-41. PMID: 28226180
9. A33 antibody-functionalized exosomes for targeted delivery of doxorubicin against colorectal cancer.  |  Li, Y., et al. 2018. Nanomedicine. 14: 1973-1985. PMID: 29935333
10. Serological responses to smallpox A33 antigen and monkeypox A35 antigen in healthy people and people living with HIV-1 from Guangzhou, China.  |  Dai, J., et al. 2023. J Med Virol. 95: e28763. PMID: 37212313
11. The human A33 antigen is a transmembrane glycoprotein and a novel member of the immunoglobulin superfamily.  |  Heath, JK., et al. 1997. Proc Natl Acad Sci U S A. 94: 469-74. PMID: 9012807
12. Characterization of posttranslational modifications of human A33 antigen, a novel palmitoylated surface glycoprotein of human gastrointestinal epithelium.  |  Ritter, G., et al. 1997. Biochem Biophys Res Commun. 236: 682-6. PMID: 9245713

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-A33 Antibody (E-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-A33 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

A33 Antibody (E-8) Specifications

Alternate Product Name: A33

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-319

Epitope Target: A33

Epitope Species: mouse

Product Reactivity/Detection: A33

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for A33 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin