α2B-AR Double Nickase Plasmid (h): sc-403430-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α2B-AR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α2B-AR Double Nickase Plasmid (h) and α2B-AR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADRA2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α2B-AR Antibody (G-9): sc-390430
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α2B-AR Double Nickase Plasmid (h) | sc-403430-NIC | 20 µg | $410.00 | |||
α2B-AR Double Nickase Plasmid (h2) | sc-403430-NIC-2 | 20 µg | $410.00 |
ADRA2B encodes the human alpha2B-adrenergic receptor (α2B-AR), a Gi/o-coupled GPCR that responds to catecholamines to modulate adenylyl cyclase activity, intracellular cAMP, and downstream PKA-dependent signaling. Receptor activation can also engage MAPK/ERK and PI3K-linked pathways through G protein and β-arrestin mechanisms, shaping cellular responses such as neurotransmitter release, smooth muscle contractility, and vascular tone. In immune and metabolic contexts, α2B-AR signaling influences stress-responsive transcriptional programs and cross-talk with other neuromodulatory receptors. Genetic and expression variation in ADRA2B has been investigated in relation to neurobehavioral phenotypes and cardiovascular regulation, supporting its relevance for mechanistic studies of adrenergic signaling networks.
α2B-AR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADRA2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADRA2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADRA2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADRA2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.