α2A-AR CRISPR/Cas9 KO Plasmid (m): sc-419023
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α2A-AR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α2A-AR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α2A-AR CRISPR/Cas9 KO Plasmid (m) | sc-419023 | 20 µg | $397.00 |
Overview
Adra2a encodes the mouse α2A-AR, a Gi/Go-coupled adrenergic receptor that responds to norepinephrine and epinephrine to inhibit adenylyl cyclase and reduce intracellular cAMP. Through GPCR signaling, α2A-AR modulates ion channel activity, presynaptic neurotransmitter release, and downstream PKA-dependent phosphorylation programs that shape synaptic transmission and neuronal excitability. In peripheral tissues, α2A-AR contributes to regulation of sympathetic tone and catecholamine-controlled cellular responses. Dysregulated adrenergic signaling involving ADRA2A has been linked to neurobehavioral phenotypes and cardiometabolic traits, making this receptor relevant to studies of stress-axis regulation and autonomic physiology.
α2A-AR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adra2a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adra2a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adra2a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α2A-AR protein expression.
This CRISPR knockout system enables efficient generation of Adra2a-deficient cell models for investigation of α2A-AR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adra2a exon(s) critical for α2A-AR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adra2a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α2A-AR CRISPR/Cas9 KO Plasmid (m) and α2A-AR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adra2a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α2A-AR HDR Plasmid (m) and α2A-AR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adra2a homology arms to support homology-directed repair at defined Adra2a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.