Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XPF Double Nickase Plasmid (h): sc-401692-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XPF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XPF Double Nickase Plasmid (h) and XPF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERCC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XPF Antibody (F-11): sc-398032
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPF Double Nickase Plasmid (h) | sc-401692-NIC | 20 µg | $410.00 | |||
XPF Double Nickase Plasmid (h2) | sc-401692-NIC-2 | 20 µg | $410.00 |
ERCC4 encodes the structure-specific endonuclease XPF, which forms a heterodimer with ERCC1 to catalyze 5′ incisions at DNA lesions and branched DNA intermediates. This complex is essential for nucleotide excision repair (NER) of bulky adducts, participates in interstrand crosslink repair within the Fanconi anemia network, and contributes to processing of stalled replication forks. XPF activity helps maintain genome stability by coordinating lesion recognition, incision, and downstream repair synthesis steps. Defects in ERCC4 function are linked to impaired DNA damage responses and are associated with UV sensitivity and genomic instability phenotypes relevant to cancer biology and inherited DNA repair disorders.
XPF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERCC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERCC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERCC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERCC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.