Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XBP1 Double Nickase Plasmid (m): sc-423727-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XBP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XBP1 Double Nickase Plasmid (m) and XBP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Xbp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XBP1 Antibody (F-4): sc-8015
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XBP1 Double Nickase Plasmid (m) | sc-423727-NIC | 20 µg | $410.00 | |||
XBP1 Double Nickase Plasmid (m2) | sc-423727-NIC-2 | 20 µg | $410.00 |
Xbp1 encodes XBP1, a basic leucine zipper transcription factor that functions as a central effector of the unfolded protein response (UPR) downstream of IRE1α-mediated mRNA splicing. Active XBP1 drives transcriptional programs that expand endoplasmic reticulum folding capacity, regulate ER-associated degradation, and reshape lipid biosynthesis to restore proteostasis during secretory stress. In mouse systems, XBP1 is widely used to study differentiation and function of professional secretory cells, metabolic adaptation, and inflammatory signaling crosstalk in tissues experiencing chronic ER stress. Dysregulated XBP1 activity has been implicated in models of metabolic disease, neurodegeneration, and immune-mediated pathology through persistent UPR signaling and altered cytokine and survival pathways.
XBP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Xbp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Xbp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Xbp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Xbp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.