WTAP Double Nickase Plasmid (h): sc-403767-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WTAP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WTAP Double Nickase Plasmid (h) and WTAP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WTAP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WTAP Antibody (D-7): sc-374280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WTAP Double Nickase Plasmid (h) | sc-403767-NIC | 20 µg | $410.00 | |||
WTAP Double Nickase Plasmid (h2) | sc-403767-NIC-2 | 20 µg | $410.00 |
WTAP (Wilms tumor 1–associated protein) is a core component of the mRNA N6-methyladenosine (m6A) “writer” complex with METTL3 and METTL14, coordinating co-transcriptional RNA methylation that influences pre-mRNA splicing, nuclear export, translation, and transcript stability. Through regulation of cell-cycle and differentiation-associated transcripts, WTAP contributes to RNA processing programs that shape proliferation and stress responses. Dysregulated WTAP expression or m6A pathway activity has been linked to altered gene expression networks in multiple cancers and developmental contexts, making it a widely studied node in epitranscriptomic control. Functional perturbation of WTAP is therefore used to interrogate m6A-dependent mechanisms governing transcript fate and cellular phenotype.
WTAP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WTAP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WTAP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WTAP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WTAP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.