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WTAP Antibody (C-12) - Western Blotting - Image 375488
WTAP Antibody (C-12): sc-166931. Western blot analysis of WTAP expression in Jurkat (A), CCRF-CEM (B), HEL 92.1.7 (C), TK-1 (D) and c4 (E) whole cell lysates and rat thymus tissue extract (F).
WTAP Antibody (C-12): sc-166931. Western blot analysis of WTAP expression in Jurkat (A), CCRF-CEM (B), HEL 92.1.7 (C), TK-1 (D) and c4 (E) whole cell lysates and rat thymus tissue extract (F).

WTAP Antibody (C-12): sc-166931

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Datasheets
  • WTAP Antibody (C-12) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 91-201 mapping near the C-terminus of WTAP of human origin
  • recommended for detection of WTAP of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for WTAP Antibody (C-12) for WB applications. These reagents are now offered in bundles with WTAP Antibody (C-12) (see ordering information below).
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    WTAP Antibody (C-12) is a mouse monoclonal IgG1 antibody that detects Wilms′ tumor protein (WTAP) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WTAP is a crucial nuclear protein that plays a significant role in regulating mRNA splicing and cell cycle progression, particularly during G2/M transition. WTAP interacts with Wilms′ tumor protein (WT1), known for functioning as a transcriptional repressor, and this interaction maintains proper gene expression and cellular function. WTAP is predominantly located in nuclear speckles, where WTAP binds to the 3′ untranslated region of cyclin A2, enhancing cyclin A2 stability and influencing cell cycle dynamics. WTAP′s role in regulating cyclin A2 levels underscores WTAP′s potential involvement in tumorigenesis, as dysregulation of cell cycle proteins can lead to uncontrolled cell proliferation. WTAP has been shown to inhibit WT1 target gene expression and can impair WT1′s ability to bind DNA, further highlighting WTAP′s regulatory significance in cellular processes. With two isoforms resulting from alternative splicing, WTAP′s functional diversity adds another layer of complexity to cellular regulation.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    WTAP Antibody (C-12) References:

    1. A role for the Wilms' tumor protein WT1 in organ development.  |  Scholz, H. and Kirschner, KM. 2005. Physiology (Bethesda). 20: 54-9. PMID: 15653840
    2. Wilms' tumor protein Wt1 regulates the Interleukin-10 (IL-10) gene.  |  Sciesielski, LK., et al. 2010. FEBS Lett. 584: 4665-71. PMID: 20974136
    3. Active specific immunotherapy targeting the Wilms' tumor protein 1 (WT1) for patients with hematological malignancies and solid tumors: lessons from early clinical trials.  |  Van Driessche, A., et al. 2012. Oncologist. 17: 250-9. PMID: 22291091
    4. Immunolocalization of Wilms' Tumor protein (WT1) in developing human peripheral sympathetic and gastroenteric nervous system.  |  Parenti, R., et al. 2014. Acta Histochem. 116: 48-54. PMID: 23791475
    5. Wilms' tumor protein induces an epithelial-mesenchymal hybrid differentiation state in clear cell renal cell carcinoma.  |  Sampson, VB., et al. 2014. PLoS One. 9: e102041. PMID: 25025131
    6. Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence.  |  Hashimoto, H., et al. 2014. Genes Dev. 28: 2304-13. PMID: 25258363
    7. The Wilms tumor protein Wt1 contributes to female fertility by regulating oviductal proteostasis.  |  Nathan, A., et al. 2017. Hum Mol Genet. 26: 1694-1705. PMID: 28334862
    8. Wilms' Tumor Protein 1 and Enzymatic Oxidation of 5-Methylcytosine in Brain Tumors: Potential Perspectives.  |  Ramsawhook, A., et al. 2018. Front Cell Dev Biol. 6: 26. PMID: 29623275
    9. Differential Expression of Wilms' Tumor Protein in Diffuse Intrinsic Pontine Glioma.  |  Lee, S., et al. 2019. J Neuropathol Exp Neurol. 78: 380-388. PMID: 30990879
    10. In vitro expansion of Wilms' tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells.  |  van der Heijden, S., et al. 2023. STAR Protoc. 4: 102053. PMID: 36853720

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    WTAP Antibody (C-12)

    sc-166931
    200 µg/ml
    		$322.00

    WTAP Antibody (C-12): m-IgG Fc BP-HRP Bundle

    sc-540177
    200 µg Ab; 10 µg BP
    		$361.00

    WTAP Antibody (C-12): m-IgG1 BP-HRP Bundle

    sc-541951
    200 µg Ab; 20 µg BP
    		$361.00

    What is the recommended fixation method for immunofluorescence staining with WTAP (C-12): sc-166931 monoclonal antibody?

    Asked by: jerojero
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 1 out of 5 by from I tested the antibody on Western blot withI tested the antibody on Western blot with three different siRNAs against WTAP (verfied with qPCR - RNA levels were below 10%) and WTAP overexpression (wildtype and GFP tagged) in MCF7 cells. The antibody only recognized the overexpressed proteins but not the endogenous WTAP. However, the dominant band on the blot was unspecific at around 40kDa. I would not recommend this antibody for Western blot.
    Date published: 2015-06-15
    Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
    Date published: 2015-02-17
    Rated 5 out of 5 by from Nice Western Blot data of WTAP expressionNice Western Blot data of WTAP expression in Jurkat and K-562 nuclear extracts. -SCBT QC
    Date published: 2014-09-02
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    WTAP Antibody (C-12) is rated 3.7 out of 5 by 3.
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