WAVE1 CRISPR Activation Plasmid (h): sc-401913-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WAVE1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- WAVE1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by WAVE1 CRISPR Activation Plasmid (h) and WAVE1 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the WASF1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WAVE1 Antibody (E-2): sc-271507
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WAVE1 CRISPR Activation Plasmid (h) | sc-401913-ACT | 20 µg | $397.00 | |||
WAVE1 CRISPR Activation Plasmid (h2) | sc-401913-ACT-2 | 20 µg | $397.00 |
WASF1 encodes WAVE1, a core component of the WAVE regulatory complex that links Rac1 signaling to Arp2/3-mediated actin nucleation and branched filament assembly. WAVE1 coordinates lamellipodia formation, membrane ruffling, and neurite outgrowth, supporting cell migration and synaptic architecture through dynamic cytoskeletal remodeling. This pathway intersects with PI3K/Rho GTPase networks and adhesion signaling to shape cellular polarity and trafficking. Dysregulation of WAVE1-associated actin control has been implicated in neurodevelopmental and neuropsychiatric phenotypes, and altered cytoskeletal signaling is also studied in invasive behavior and metastatic cell biology contexts.
WAVE1 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous WASF1 expression without altering the underlying DNA sequence.
WAVE1 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the WASF1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the WASF1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous WAVE1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native WASF1 locus and enabling the study of WAVE1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of WAVE1 pathway restoration in tumor cells with silenced or reduced WASF1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.