WAPL Double Nickase Plasmid (m): sc-432331-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WAPL Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WAPL Double Nickase Plasmid (m) and WAPL Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Wapl. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WAPL Double Nickase Plasmid (m) | sc-432331-NIC | 20 µg | $410.00 | |||
WAPL Double Nickase Plasmid (m2) | sc-432331-NIC-2 | 20 µg | $410.00 |
Mouse Wapl encodes WAPL, a cohesin-release factor that promotes opening of the cohesin ring and regulates residence time of cohesin on chromatin. Through control of cohesin dynamics, WAPL influences sister chromatid cohesion, mitotic progression, DNA replication timing, and higher-order genome organization that shapes transcriptional programs. WAPL function interfaces with pathways governing chromosome segregation, DNA damage responses, and chromatin loop formation, making it a key node in genome stability. Dysregulated cohesin turnover and altered WAPL activity have been associated with aneuploidy, replication stress, and cohesinopathy-related developmental phenotypes, supporting its relevance in models of disease biology.
WAPL Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Wapl locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Wapl. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Wapl function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Wapl-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.