Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Villin CRISPR/Cas9 KO Plasmid (h): sc-400587
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Villin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Villin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Villin Antibody (1D2C3): sc-58897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Villin CRISPR/Cas9 KO Plasmid (h) | sc-400587 | 20 µg | $397.00 |
Overview
VIL1 encodes villin, an epithelial-specific actin-binding protein enriched in the brush border of intestinal and renal proximal tubule cells, where it bundles, caps, and severs F-actin to shape microvilli architecture. Through Ca2+- and phosphoinositide-regulated actin remodeling, villin supports epithelial polarity, membrane trafficking at the apical surface, and barrier function during differentiation and regeneration. VIL1 is widely used as a marker of enterocyte lineage and epithelial maturation, and altered villin expression or localization is reported in gastrointestinal injury and inflammatory contexts as well as epithelial tumor biology. These properties make VIL1 a practical node for studying cytoskeletal dynamics, epithelial morphogenesis, and microvillar maintenance.
Villin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VIL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VIL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VIL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Villin protein expression.
This CRISPR knockout system enables efficient generation of VIL1-deficient cell models for investigation of Villin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VIL1 exon(s) critical for Villin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VIL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Villin CRISPR/Cas9 KO Plasmid (h) and Villin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VIL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Villin HDR Plasmid (h) and Villin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VIL1 homology arms to support homology-directed repair at defined VIL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.