USP51 Double Nickase Plasmid (h): sc-414805-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP51 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP51 Double Nickase Plasmid (h) and USP51 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP51. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP51 Double Nickase Plasmid (h) | sc-414805-NIC | 20 µg | $410.00 | |||
USP51 Double Nickase Plasmid (h2) | sc-414805-NIC-2 | 20 µg | $410.00 |
Human USP51 encodes a deubiquitinating enzyme that cleaves ubiquitin from protein substrates, contributing to ubiquitin-dependent control of protein stability, subcellular localization, and signaling duration. As part of the ubiquitin–proteasome regulatory network, USP51 is studied in pathways that coordinate DNA damage responses, chromatin-associated processes, and cell-cycle progression through dynamic ubiquitination and deubiquitination. Perturbation of deubiquitinase activity can rewire proteostasis and stress-response signaling, making USP51 a relevant node for investigating mechanisms underlying altered genome maintenance and transcriptional programs in disease-associated cellular states. Functional interrogation of USP51 supports research into how ubiquitin editing modulates pathway crosstalk and phenotypes linked to dysregulated protein turnover.
USP51 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP51 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP51. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP51 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP51-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.