USP2 Double Nickase Plasmid (h): sc-411243-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP2 Double Nickase Plasmid (h) and USP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP2 Double Nickase Plasmid (h) | sc-411243-NIC | 20 µg | $410.00 | |||
USP2 Double Nickase Plasmid (h2) | sc-411243-NIC-2 | 20 µg | $410.00 |
Human USP2 encodes ubiquitin-specific peptidase 2, a deubiquitinating enzyme that removes ubiquitin chains from substrate proteins to regulate their stability, localization, and signaling output. USP2 participates in ubiquitin-dependent proteostasis and modulates pathways controlling cell-cycle progression, apoptosis, DNA damage responses, and inflammatory signaling through selective deubiquitination of pathway components. By shaping turnover of key regulators, USP2 influences cellular stress adaptation and metabolic homeostasis, and its dysregulation has been studied in contexts such as tumor biology, immune signaling imbalance, and neurodegenerative protein aggregation. These properties make USP2 a useful node for interrogating ubiquitin circuitry and signaling cross-talk in human cells.
USP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.