UQCRC1 CRISPR/Cas9 KO Plasmid (m): sc-423625
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UQCRC1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UQCRC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UQCRC1 CRISPR/Cas9 KO Plasmid (m) | sc-423625 | 20 µg | $397.00 |
Overview
Uqcrc1 encodes ubiquinol-cytochrome c reductase core protein 1 (UQCRC1), an essential structural component of mitochondrial respiratory chain complex III (cytochrome bc1) that supports electron transfer from ubiquinol to cytochrome c and helps sustain oxidative phosphorylation. By influencing mitochondrial membrane potential, ATP production, and reactive oxygen species homeostasis, UQCRC1 contributes to cellular energy metabolism and redox signaling programs. Perturbation of complex III function can disrupt metabolic adaptation and stress responses, processes frequently interrogated in models of neurodegeneration, cardiometabolic phenotypes, and proliferative disease biology. As a core complex III subunit, UQCRC1 is commonly studied in pathways linking mitochondrial dysfunction to altered apoptosis sensitivity, hypoxic signaling, and integrated cellular bioenergetics.
UQCRC1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Uqcrc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Uqcrc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Uqcrc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UQCRC1 protein expression.
This CRISPR knockout system enables efficient generation of Uqcrc1-deficient cell models for investigation of UQCRC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Uqcrc1 exon(s) critical for UQCRC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Uqcrc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UQCRC1 CRISPR/Cas9 KO Plasmid (m) and UQCRC1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Uqcrc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UQCRC1 HDR Plasmid (m) and UQCRC1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Uqcrc1 homology arms to support homology-directed repair at defined Uqcrc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.