UFSP2 Double Nickase Plasmid (h): sc-408911-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UFSP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UFSP2 Double Nickase Plasmid (h) and UFSP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UFSP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UFSP2 Antibody (G-11): sc-376084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFSP2 Double Nickase Plasmid (h) | sc-408911-NIC | 20 µg | $410.00 | |||
UFSP2 Double Nickase Plasmid (h2) | sc-408911-NIC-2 | 20 µg | $410.00 |
UFSP2 encodes UFM1-specific peptidase 2, a cysteine protease that processes UFM1 to its mature form and removes UFM1 modifications from substrate proteins, thereby regulating the UFMylation cycle. This activity supports proteostasis pathways linked to endoplasmic reticulum homeostasis, ribosome-associated quality control, and stress-responsive signaling that influence protein translation and secretion. UFSP2-dependent UFMylation dynamics have been connected to hematopoietic and immune cell function, and altered regulation of this pathway has been implicated in human disease contexts where ER stress and protein quality control are disrupted. As a research target, UFSP2 is used to dissect how deUFMylation controls substrate turnover and stress adaptation across diverse cell types.
UFSP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UFSP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UFSP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UFSP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UFSP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.