Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UDP-GlcDH CRISPR/Cas9 KO Plasmid (m): sc-423601
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UDP-GlcDH CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UDP-GlcDH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UDP-GlcDH Antibody (B-4): sc-137058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UDP-GlcDH CRISPR/Cas9 KO Plasmid (m) | sc-423601 | 20 µg | $397.00 |
Overview
Mouse Ugdh encodes UDP-glucose 6-dehydrogenase (UDP-GlcDH), a cytosolic enzyme that oxidizes UDP-glucose to UDP-glucuronic acid, a key precursor for glycosaminoglycan and proteoglycan biosynthesis. This reaction supports extracellular matrix assembly and remodeling by supplying substrates for hyaluronan and chondroitin/dermatan sulfate production, influencing cell adhesion, migration, and tissue morphogenesis. Ugdh activity also connects carbohydrate metabolism to UDP-sugar interconversion pathways that shape glycocalyx composition and cell–cell signaling. Dysregulated UDP-glucuronic acid availability and matrix glycosylation have been linked to altered developmental programs, fibrosis-like matrix changes, and cancer-associated invasion phenotypes in experimental models.
UDP-GlcDH CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ugdh gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ugdh together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ugdh open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UDP-GlcDH protein expression.
This CRISPR knockout system enables efficient generation of Ugdh-deficient cell models for investigation of UDP-GlcDH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ugdh exon(s) critical for UDP-GlcDH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ugdh genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UDP-GlcDH CRISPR/Cas9 KO Plasmid (m) and UDP-GlcDH CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ugdh locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UDP-GlcDH HDR Plasmid (m) and UDP-GlcDH HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ugdh homology arms to support homology-directed repair at defined Ugdh target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.