Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UBE2F Double Nickase Plasmid (h): sc-406582-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2F Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBE2F Double Nickase Plasmid (h) and UBE2F Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBE2F. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UBE2F Antibody (C-11): sc-398668
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2F Double Nickase Plasmid (h) | sc-406582-NIC | 20 µg | $410.00 | |||
UBE2F Double Nickase Plasmid (h2) | sc-406582-NIC-2 | 20 µg | $410.00 |
Human UBE2F encodes an E2-conjugating enzyme that channels NEDD8 to cullin substrates, supporting assembly and regulation of cullin–RING ligases and downstream ubiquitin-dependent proteostasis. By shaping the neddylation state of specific cullins, UBE2F influences turnover of key regulators of cell-cycle progression, stress responses, and DNA damage signaling, thereby impacting transcriptional programs and cellular homeostasis. Dysregulated ubiquitin–proteasome and neddylation pathways are frequently implicated in oncogenic transformation and altered stress tolerance, making UBE2F a useful node for mechanistic studies of protein quality control and signaling crosstalk. Functional interrogation of UBE2F can help clarify how selective cullin neddylation biases substrate recognition and proteolytic outputs in human cells.
UBE2F Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBE2F locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBE2F. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBE2F function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBE2F-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.