Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TSG-6 Double Nickase Plasmid (h): sc-400765-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSG-6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TSG-6 Double Nickase Plasmid (h) and TSG-6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNFAIP6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TSG-6 Antibody (E-1): sc-377277
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSG-6 Double Nickase Plasmid (h) | sc-400765-NIC | 20 µg | $410.00 | |||
TSG-6 Double Nickase Plasmid (h2) | sc-400765-NIC-2 | 20 µg | $410.00 |
TNFAIP6 encodes tumor necrosis factor-stimulated gene-6 (TSG-6), a secreted hyaladherin induced by inflammatory cytokines that remodels the extracellular matrix through interactions with hyaluronan and the heavy chains of inter-α-inhibitor. By catalyzing and stabilizing hyaluronan–heavy chain complexes and modulating protease activity, TSG-6 influences leukocyte trafficking, tissue edema, and wound-associated matrix organization. TNFAIP6 expression is linked to NF-κB-driven inflammatory programs and microenvironmental cues that shape stromal–immune communication. Dysregulated TSG-6 biology has been associated with chronic inflammatory states and tumor-associated stroma, making it a relevant node for mechanistic studies of matrix–inflammation crosstalk.
TSG-6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNFAIP6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNFAIP6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNFAIP6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNFAIP6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.