Date published: 2026-5-5

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Thy-1/CD90 Antibody (FF-10): sc-59399

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Datasheets
  • Thy-1/CD90 Antibody (FF-10) is a rat monoclonal IgG1 provided at 200 µg/ml
  • raised against EL-4 cells of mouse origin
  • recommended for detection of Thy-1 on T cell lines (EL 4, 3A9), T lymphocytes, the WEH1-3 cell line and 3T3 fibroblasts of mouse origin by WB, IP, IF and FCM
  • See Thy-1/CD90 (aTHy-1A1): sc-53456 for Thy-1/CD90 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Thy-1/CD90 Antibody (FF-10). This work is in progress.
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    Thy-1 Antibody (FF-10) is a rat monoclonal IgG1 antibody that detects Thy-1 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Thy-1, also known as CDw90, is a phosphatidyl-anchored cell surface glycoprotein that plays a crucial role in cell adhesion and signaling, particularly in the context of hematopoietic stem cells. Expression of Thy-1 is vital for identifying specific subpopulations of cells within mixed colonies, especially when coexpressed with CD34, as Thy-1 marks a subset of putative hematopoietic, pluripotent stem cells in normal human bone marrow. Thy-1+ cells have been implicated in syngeneic graft versus host disease, highlighting Thy-1′s importance in regulating autoreactivity following bone marrow transplants. Understanding Thy-1 function is essential for advancing therapeutic strategies in regenerative medicine and immunology, as Thy-1 may influence stem cell behavior and interactions within the hematopoietic niche. Anti-Thy-1 antibody (FF-10) serves as an invaluable tool for researchers investigating these complex biological processes.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Thy-1/CD90 Antibody (FF-10)

    sc-59399
    200 µg/ml
    $322.00

    What blocking solution should I use with Thy-1 (FF-10): sc-59399 monoclonal antibody for Western blot?

    Asked by: TinTin
    Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Robust signal in WB in mouse PBL whole cell lysateRobust signal in WB in mouse PBL whole cell lysate. -SCBT QC
    Date published: 2015-04-10
    Rated 5 out of 5 by from Good specificity in WB in mouse brain tissueGood specificity in WB in mouse brain tissue extract. -SCBT QC
    Date published: 2014-10-23
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    Thy-1/CD90 Antibody (FF-10) is rated 5.0 out of 5 by 2.
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