THUMPD3 CRISPR/Cas9 KO Plasmid (m): sc-420731
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- THUMPD3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the THUMPD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
THUMPD3 CRISPR/Cas9 KO Plasmid (m) | sc-420731 | 20 µg | $397.00 |
Overview
Thumpd3 encodes THUMPD3, a THUMP domain–containing protein implicated in RNA-binding functions that support RNA metabolism and post-transcriptional regulation. THUMP domains are commonly associated with tRNA/rRNA modification enzymes and are linked to processes that influence ribosome biogenesis, translation fidelity, and cellular stress responses. In mouse systems, perturbation of RNA processing and translational control can impact proliferation and differentiation programs, making THUMPD3 relevant for studying gene expression homeostasis. Altered RNA modification and processing pathways are broadly connected to phenotypes in neurodevelopment, metabolic regulation, and cancer biology, providing mechanistic context for investigating THUMPD3-dependent networks.
THUMPD3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Thumpd3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Thumpd3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Thumpd3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish THUMPD3 protein expression.
This CRISPR knockout system enables efficient generation of Thumpd3-deficient cell models for investigation of THUMPD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Thumpd3 exon(s) critical for THUMPD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Thumpd3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by THUMPD3 CRISPR/Cas9 KO Plasmid (m) and THUMPD3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Thumpd3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by THUMPD3 HDR Plasmid (m) and THUMPD3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Thumpd3 homology arms to support homology-directed repair at defined Thumpd3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.