Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Thrombospondin 5 Double Nickase Plasmid (h): sc-402797-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Thrombospondin 5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Thrombospondin 5 Double Nickase Plasmid (h) and Thrombospondin 5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COMP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Thrombospondin 5 Antibody (F-7): sc-374660
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thrombospondin 5 Double Nickase Plasmid (h) | sc-402797-NIC | 20 µg | $410.00 | |||
Thrombospondin 5 Double Nickase Plasmid (h2) | sc-402797-NIC-2 | 20 µg | $410.00 |
COMP encodes thrombospondin 5, a secreted pentameric extracellular matrix glycoprotein abundant in cartilage and other connective tissues. Thrombospondin 5 participates in matrix assembly and homeostasis by binding collagens, proteoglycans, and integrins, thereby influencing chondrocyte adhesion, mechanotransduction, and extracellular matrix organization. Through its effects on cartilage architecture and endochondral ossification-related processes, COMP is linked to pathways governing skeletal development and tissue integrity. Genetic disruption or dysregulation of COMP is associated with inherited skeletal dysplasias and cartilage degeneration phenotypes, making it a relevant target for studying extracellular matrix biology and connective tissue disease mechanisms.
Thrombospondin 5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COMP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COMP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COMP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COMP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.